Tensometric characteristics of the protective effect of a novel quinazolinone derivative on ischemic muscle contraction
O. Mykhailenko, O. Lehedza, N. Semenuk, D. Zavodovskyi
Abstract: Objective: To conduct a basic investigation into the efficacy of a new quinazolinone derivative, henceforth referred to as Compound Q, in preserving skeletal muscle contractile function following severe 3-hour ischemic injury in a rat model. Materials and methods: Wistar rats were surgically induced with 3-hour hind limb ischemia. Compound Q was administered intramuscularly at a dose of 10 mg/kg 30 minutes before the onset of ischemia. The functional capacity of the calf muscle was assessed in situ using tensiometry during a protocol of repeated high-frequency electrical transnervous stimulation. Results: A three-hour ischemia period led to substantial functional impairment, evidenced by a contraction amplitude reduction of over 92% and a total work decrease of 82.4%. Pretreatment with Compound Q led to a significant attenuation of this decline. Specifically, the fatigue rate was found to be 6-10 times lower than that observed in the ischemic group. However, an analysis of the force curves revealed a significant impairment of the muscle's ability to relax completely. Discussion: The preservation of the mechanokinetic characteristics of muscle function indicates that Compound Q effectively counteracts key pathological events of ischemic-reperfusion injury, probably due to its antioxidant and anti-inflammatory effects. Impaired relaxation signifies a partial protective effect in which energy-dependent processes, such as sarcoplasmic/endoplasmic reticulum Ca²⁺-ATPase (SERCA) function, remain compromised. Conclusion: Compound Q, which was proposed and synthesized by the authors, is a promising therapeutic candidate for the protection of contractile muscle function in ischemic injury. Further comprehensive investigation is required to determine its efficacy.
Series on Biomechanics, Vol.39, No.1(2025), 48-53
DOI: 10.7546/SB.05.03.2025
Keywords: ischemic damage; mechanogram; quinazolinone; skeletal muscle; tensiometry
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| Date published: 2025-10-28
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