Innovative AC phase shift technique for rapid and precise blood clotting time measurement
R. Zlatev
, N. Antonova
, M. Stoytcheva
, R.Ramos
Abstract: Objective: This study aims to introduce and validate an innovative AC phase shift technique for rapid and precise measurement of blood clotting time, offering a potential improvement over traditional coagulation testing methods. Materials and Methods: Blood samples were mixed with a 2% CaCl2 solution to induce coagulation. Using a virtual measurement setup and a modified viscometer, AC current phase shift was recorded across a frequency range of 1 Hz to 10 kHz. The phase shift data were compared with shear stress measurements to evaluate performance. Results: The AC phase shift method effectively tracked coagulation with high precision. Significant phase shifts were observed at 1000 Hz, where the method distinguished the coagulation time as 7 minutes and 15 seconds. In comparison, shear stress measurements showed less clear delineation of coagulation onset. Discussion: The AC phase shift method demonstrated superior sensitivity and accuracy, revealing distinct phase shift changes corresponding to the coagulation process. This technique provided a clearer and more consistent measurement compared to traditional rheological methods, which showed variability in viscosity changes. Conclusion: The AC phase shift technique offers a promising alternative for blood coagulation assessment, with high precision and real-time monitoring capabilities. This approach could enhance diagnostic accuracy and efficiency in clinical settings.
Series on Biomechanics, Vol.38, No.4(2024),41-47
DOI: 10.7546/SB.06.04.2024
Keywords: AC phase shift; blood coagulation; shear stress measurements
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| Date published: 2024-12-11
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