The usefulness of red blood cell osmotic gradient deformability (osmoscan) measurements in surgical research: A single-center experience
A. Varga
, A.A. Matrai
, N. Nemeth
Резюме: Objective: Red blood cell (RBC) deformability is an essential micro-rheological parameter playing an important role in blood flow, especially in microcirculation. In the last 15 years, we collected experiences in methodological standardization, comparative hemorheology and experimental and clinical research using the osmotic gradient deformability (osmoscan) method. In this paper we aim to overview the main findings, focusing on the information content of the osmoscan parameters in various pathophysiological processes. Materials and Methods: The conventional ektacytometry test describes RBCs’ elongation index in the function of shear stress, while the osmoscan tests are performed in the function of osmolality at a constant shear stress. The result is the well-defined elongation index (EI) – osmolality (O) curve. The EI-O curve can be characterized by several calculated parameters that show alteration in various pathophysiological processes. Results: The magnitude of changes in conventional and osmoscan deformability values can be different from each other, depending on the determinant noxa (e.g., damage by free radicals, cell-structure/morphology alterations, mechanical, thermal or metabolic effects, etc.). Discussion: Red blood cell osmotic gradient deformability measurement provides much more detailed information about the deformability of the erythrocytes, being useful in various research (both clinical and experimental), focusing on micro-rheological alterations. The standard settings of the measurement conditions are highly important to have comparable results. Conclusion: The presented experiences may call attention to the usefulness of parallel investigation of conventional and osmotic gradient deformability.
Series on Biomechanics, Vol.39, No.1 (2025), 3-8
DOI: 10.7546/SB.01.01.2025
Ключови думи: Hemorheology; osmotic gradient deformability; red blood cell deformability; surgical research
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| Дата на публикуване: 2025-03-25
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