Modulation of myometrium contractive activity and intracellular Са2 homeostasis by calix4arene С-1130 selective inhibitor of the sodium pump
O. Tsymbalyuk
, T. Veklich
, R. Rodik
, O. Maliuk
, S. Karakhim
, V. Kalchenko
, S. Kosterin
Резюме: Objective: Na+,K+-ATPase of the plasma membrane plays an important role in maintaining the excitability of smooth muscle cells; directed modulation of the activity of this enzyme can be used to normalize the tone and contractility of muscle tissue. Materials and methods: The cone-like dioctyloxycalix[4]arene C-1130, containing two bioaffine formations of (2-pyridyl)methylaminophosphonic acid on the upper rim of the macrocycle, was syn-thesized. The methods of enzymatic and kinetic analysis were used for the study of the AТР-hydrolase activities in plasma membranes of the rat myometrium cells. Determining the changes in the concentration of intracellular Са2+ was studied with confocal microscopy. The study of the spontaneous contractive activity (registered in the isomet-ric and isotonic modes) in muscle preparations was conducted according to the method of complex mechanokinetic analysis. Results: Calix[4]arene С-1130 had an efficient inhibitory effect on Na+, K+-ATPase activity in the plasma membrane of myocytes (І0.5 = 38 ± 6 nM), and had no effect on other ATPases. С-1130 (10 µM) conditioned the increase in the intracellular concentration of Са ions in the myocytes and activated the spontaneous contractive activity of rat myometrium. This calix[4]arene changed time, force, and velocity parameters of isometric and iso-tonic spontaneous contractions. Under the preliminary effect of ouabain, C-1130 did not change any mechanoki-netic parameter of spontaneous contractions. Discussion: Calix[4]arene С-1130 only blocked Na+,К+-ATPase of the plasma membrane without affecting other proteins, involved in the processes of myometrium contraction-relaxation. Conclusion: Calix[4]arene С-1130, a selective inhibitor of Nа+, К+-ATPase, may be relevant for the creation of medicines and for the pharmacological researches.
Series on Biomechanics, Vol.38, No.1 (2024), 71-89
DOI: 10.7546/SB.08.01.2024
Ключови думи: calix4arenes; contrac-tion; mechanokinetics; myometrium; Nа; plasma membrane; smooth muscle cells; К-ATPase
| Литература: (click to open/close) | [1] Arakawa, T. K., Mlynarczyk, M., Kaushal, K. M., Zhang, L., Ducsay, C. A., 2004. Long-term hypoxia alters calcium regulation in near-term ovine myometrium. Biology of reproduction 71,1, 156–162.
[2] Buxton, I.L., 2004. Regulation of uterine function: a biochemical conundrum in the regulation of smooth muscle relaxation. Molecular Pharmacology 65. 5, 1051-1059.
[3] Woodcock, N.A., Taylor, C.W., 2004. Effect of an oxytocin receptor antagonist and rho kinase inhibitor on the [Ca2+]I sensitivity of human myometrium. The American Journal of Obstetrics and Gynecology 190, 1, 222-228.
[4]Biser, P. S., Thayne, K. A., Fleming, W. W., Taylor, D. A., 2002. Na+, K+ ATPase alpha-subunit isoform distribution and abundance in guinea-pig longitudinal muscle/myenteric plexus after exposure to morphine. Brain research 93, 12, 186–193.
[5] Geering, K., 2006. FXYD proteins: new regulators of Na+,K+-ATPase. American Journal of Physiology - Renal Physiology 290, 241-250.
[6] Isenovic, E. R., Jacobs, D. B., Kedees, M. H., Sha, Q., Milivojevic, N., Kawakami, K., Gick, G., & Sowers, J. R., 2004. Angiotensin II regulation of the Na+ pump involves the phosphatidylinositol-3 kinase and p42/44 mitogen-activated protein kinase signaling pathways in vascular smooth muscle cells. Endocrinology 145, 3, 1151–1160.
[7] Jain, D., Cnhabra, S.K., Paj, H.G., 2004. Effect of sensitization on membrane ion fluxes & intracellular calcium in guinea pigs. The Indian Journal of Medical Research 120, 534-541.
[8] Santos, L., Xavier, F.E., Vassallo, D.V., Rossoni, L.V., 2003. Cyclooxygenase pathway is involved in the vascular reactivity and inhibition of the Na+,K+-ATPase activity in the tail artery from L-NAME-treated rats. Life Science. 74, 5, 613-627.
[9] Kosterin, S.O., Kalchenko, V.I., Veklich, Т.О., Babich, L.G., Shlykov, S.G., 2019. Kaliksareny yak moduliatory ATF-hidrolaznykh system v hladenkykh miazakh. Science opinion, Kyiv, Ukraine
[10] Pan, Y. C., Hu, X. Y., Guo, D. S., 2021. Biomedical Applications of Calixarenes: State of the Art and Perspectives. Angewandte Chemie (International ed. in English) 60, 6, 2768–2794.
[11] Coleman, A.W., Jebors, S., Cecillon, S., Perret, P., Garin, D., Marti-Battle, D., Moulin, M., 2008. Toxicity and biodistribution of para-sulfonato-calix[4]arene in mice. New Journal of Chemistry 32, 780-782.
[12] Rodik, R.V., Boyko, V.I., Kalchenko, V.I., 2016. Calixarenes in Biotechnology and Bio-Medical Researches. Frontiers in Medicinal Chemistry 8, 206-301.
[13] Abd Karim, N. F. N., Supian, F. L., Musa, M., Ayop, S. K., Azmi, M. S. M., Yazid, M. D., Yi, W. Y., 2023. Calixarene Derivatives: A Mini-Review on their Synthesis and Demands in Nanosensors and Biomedical Fields. Mini reviews in medicinal chemistry 23, 6, 734–745.
[14] Veklich T.O., Kosterin S.O., Rodik R.V., Cherenok S.О., Boyko V.I., Kalchenko V.I., 2006. Efekt kaliksaren-fosfonovoi kysloty na aktyvnist Na+,K+-ATF-azyv plazmatychnykh membranakh hladenkomiazovykh klityn. The Ukrainian Biochemical Journal 78, 1, 70-86.
[15] Veklich, T.O., Labyntseva, R.D., Shkrabak, O.A., Tsymbalyuk, O.V., Rodik, R.V., Kalchenko, V.I., Kosterin, S.O., 2020. Inhibuvannia Na+,K+-ATF-azy ta aktyvatsiia ATF-zy miozynu kaliks[4]arenom S-107 sprychyniaie skorochuvalnoi aktyvnosti izolovanykh hladenkykh miaziv. The Ukrainian Biochemical Journal 92, 1, 21-30.
[16] Danylovych G.V., Kolomiets О.V., Danylovych Y.V., Rodik R.V., Kаlchenko V.I., Kosterin S.О. 2018. Cаlіx[4]аrene С-956 is effective inhibitorof Н+-Сa2+-exchanger in smooth muscle mitochondria. The Ukrainian Biochemical Journal 90, 1, 25-33.
[17] Veklich T.O., Shkrabak A.A., Mazur Yu.Yu., Rodik R.V., Boyko V.I., Kalchenko V.I., Kosterin S.O., 2013. Kinetic regularities of calixarene C-90 action on the myometrial plasma membrane Ca2+, Mg2+-ATPase activity and on the Ca2+ concentration in unexcited сells of the myometrium. The Ukrainian Biochemical Journal 85, 4, 20-27.
[18] Kondratuk, Т.P., Buchenuk, S.F., Prichepa, А.А., Babich, L.H., Kurskiy, M.D., Osipenko, A.A., 1986. Allocation and characterization of membrane fraction plasma membrane myometrium. The Ukrainian Biochemical Journal 58, 4, 50-56.
[19] Veklich, Т.О., Kosterin, S.O., 2005. Porivnialne doslidzhennia vlastyvostei Na+,K+-ATF-azy ta Mg2+-ATF-azy plazmatychnykh membran miometriia. The Ukrainian Biochemical Journal 77, 2, 66-75.
[20] Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the prinsiple of protein-dye binding. Analytical Biochemistry: Methods in the Biological Sciences 72, 1-2, 248-282.
[21] Mollard, P., Mironneau, J., Amedee, T., Mironneau, C., 1986. Electrophysiological characterization of single pregnant rat myometrial cells in short-term primary culture. The American Journal of Physiology-Cell Physiology 19, 1, C47-C54.
[22] Rathbun, W., Betlach, V., 1969. Estimation of enzymically produced orthophosphate in the presence of cystein and adenosine triphosphate. Analytical Biochemistry: Methods in the Biological Sciences 28, 1-3, 436-445.
[23] Kosterin, S., Tsymbalyuk, O., Holden, O., 2021. Multiparameter analysis of mechanokinetics of the contractile response of smooth muscles. Series on Biomechanics 35, 1, 14-30.
[24] Van Hoorn, W.P., Briels, W.P., van Duynhoven, J.P.M., van Veggel, F.C.J.M., Reinhoudt, D.N., 1998. Conformational distribution of tetramethoxycalix[4]arenes by molecular modeling and NMR spectroscopy: a study of apolar solvation. The Journal of Organic Chemistry 63, 1299–1308.
[25] Kosterin, S., Tsymbalyuk, O., 2023. Mechanokinetics and power of the spontaneous isotonic contraction of visceral smooth muscles Series on Biomechanics 37, 3, 43-56.
[26] Gutsche, C.D., Dhawan, B., Levine, J.A., No, K. H., Bauer, L. J., 1983. Conformational isomers of the ethers and esters of calix[4]arenes. Tetrahedron 39, 409–426.
[27] Tsymbalyuk, O.V., 2014. Porivnialnyi analiz mekhanokinetyky skorochuvalnoi aktyvnosti miometriia shchuriv za dii kaliksarenu S-99 i uabainu. Studia Biologica. 8, 1, 63-72.
[28] Tsymbalyuk, O.V., 2016. Vplyv kaliksarenu S-99 na skorochuvalnu aktyvnist miaziv tovstoho kyshechnyka shchuriv. Studia Biologica. 10, 3-4, 33-46.
[29] Tsymbalyuk, O.V., Kosterin, S.O., Kalchenko, V.I., Rodik, R.V., 2010. Mekhanokinetychni parametry skorochuvalnoi aktyvosti hladenkykh miaziv caecum shchuriv za umov khronichnoi dii kaliksarenu S-107 in vivo. Physics alive. 18, 1, 47-51.
[30] Tsymbalyuk, O.V., Kosterin, S.O., Kalchenko, V.I., Rodik, R.V., 2010. Porivnialne doslidzhennia efektiv kaliksarenu S-107 ta uaabainu v umovakh in vitro ta in vivo na aktyvnist Na+,K+-ATF-azy v plazmatychnii membrani hepatotsytiv shchuriv. The Ukrainian Biochemical Journal 82, 4, 78-85.
[31] Matthew, A, Shmygol, A, Wray, S., 2004. Ca2+ entry, efflux and release in smooth muscle. Biological Research 37, 4, 617-24.
[32] Tsymbalyuk, O.V., Kosterin, S.O., 2012. Na+,K+-ATF-aza, endohenni kardiosteroidy ta yikh transduktorna rol. The Ukrainian Biochemical Journal 84, 1, 5-17.
[33] Noble, K., Floyd, R., Shmygol, A., Shmygol, A., Mobasheri, A., Wray, S., 2010. Distribution, expression and functional effects of small conductance Ca-activated potassium (SK) channels in rat myometrium. Cell Calcium. 47, 1, 47-54.
[34] Sadlonova, V., Franova, S., Dokus, K., Janicek, F., Visnovsky, J., Sadlonova, J., 2011. Participation of BK Ca2+ and KATP potassium ion channels in the contractility of human term pregnant myometrium in in vitro conditions. Journal of Obstetrics and Gynecology Research 37, 3, 215-21.
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| Дата на публикуване: 2024-04-23
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