Рrotective effects of two standard preservation media on irradiated erythrocytes under storage conditions: A rheological comparative evaluation
S. Porini
, B. D. Riquelme
, H. V. Castellini
, G. Detarsio
, A. Aresi
, N. Manzelli
, L. Di Tullio
, A. Acosta
, M. Galassi
, A. I. Alet
Резюме: Introduction: Transfusion units are irradiated in hemotherapy centers to prevent graft-versus-host disease by inactivating donor lymphocytes. High-energy photon radiation damages the DNA of nucleated blood cells, shortening the lifespan of erythrocytes from 35 (CPDA-1) or 42 (CPD-Optisol®) to 28 days (according to Argentine regulations). The mean corpuscular volume and free sodium and potassium levels are the most affected biochemical parameters. The preservation media commonly used during red blood cell storage are designed to protect them from this damage. Objective: The aim was to evaluate the alteration in the hemorheological properties of gamma-irradiated erythrocyte concentrates storage in two typical preservation media. Materials and Methods: Erythrocyte concentrate units preserved in CPDA-1 or CPD-Optisol® were utilized for the experiments. Each unit was divided into 6 satellite bags, 5 of which were irradiated at different doses (2, 5, 10, 15, and 25 Gy), and the remaining one was used as a control (0 Gy). Samples from each bag were analyzed weekly for 35 days. The viscoelastic and aggregation parameters of the erythrocyte were determined using the Erythrocyte Rheometer and the Optical Chip Aggregometer. Results: Hemorheological parameters exhibited different changes depending on the storage time, the gamma dose, and the specific preservation medium used. Discussion: The data obtained on the erythrocyte damages due to the radiation dose and storage are related to the different properties of the two standard preservation media evaluated. Conclusion: Results could help to design a new preservation medium for irradiated red blood cell units reducing damage caused by storage.
Series on Biomechanics, Vol.38, No.4(2024),24-31
DOI: 10.7546/SB.04.04.2024
Ключови думи: blood preservation media; blood storage; Erythrocyte irradiation; hemorheology; transfusional units
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| Дата на публикуване: 2024-12-11
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