Functional properties of leukocyte surface in patients with type 2 diabetes mellitus
M.Yu. Skorkina, N. M. Antonova, N.I. Zernakova, A.S.Alexandrova, T. Yu. Lebedev, O.V. Cherkashina
Резюме: Background: The development of pathological processes accompanying type 2 Diabetes mellitus is closely related to the structural and metabolic status of leukocytes. The functional activity of leukocytes is determined by such properties of the leukocytes’ membrane as elastic properties, surface potential, and intercellular adhesion force. Leukocytes play a key role in the development of pathogenetic reactions in the progression of type 2 Diabetes mellitus.(какво искат да кажат?)
Objective: The aim of the study was to investigate the functional properties of leukocytes' surface (mechanical, electrical and adhesive ) in patients with type 2 diabetes mellitus.
Methods: The method of atomic force microscopy (AFM) was used in the study. The electrical properties and intercellular adhesion forces of the leukocyte membrane were estimated to carry out the measure of surface potential in the Kelvin mode by atomic force spectroscopy. The stiffness of the cell surface was estimated on the numeric data of Young's module by registering force curves from the cell surface using an atomic force microscope.
Results: For the group of patients with type 2 diabetes mellitus the decreased adhesion force between erythrocyte and leukocyte by 65.5% (p<0.05) was found as compared with the control group. The adhesion force between blood cells the surface potential of leukocytes in the patients with type 2 diabetes mellitus was more negative (-58.19±0.67 mV) as compared with cells from the control group (-38.85±0.76 mV).
Conclusion: The results indicate the functional immaturity of immune cells of the patients with type 2 diabetes mellitus and give reason to consider the changes of membrane properties of leukocytes as one of the pathogenetic mechanisms of immune cells for developing hyperglycemic conditions.
Series on Biomechanics, Vol.34, No.4 (2020), 18-25
Objective: The aim of the study was to investigate the functional properties of leukocytes' surface (mechanical, electrical and adhesive ) in patients with type 2 diabetes mellitus.
Methods: The method of atomic force microscopy (AFM) was used in the study. The electrical properties and intercellular adhesion forces of the leukocyte membrane were estimated to carry out the measure of surface potential in the Kelvin mode by atomic force spectroscopy. The stiffness of the cell surface was estimated on the numeric data of Young's module by registering force curves from the cell surface using an atomic force microscope.
Results: For the group of patients with type 2 diabetes mellitus the decreased adhesion force between erythrocyte and leukocyte by 65.5% (p<0.05) was found as compared with the control group. The adhesion force between blood cells the surface potential of leukocytes in the patients with type 2 diabetes mellitus was more negative (-58.19±0.67 mV) as compared with cells from the control group (-38.85±0.76 mV).
Conclusion: The results indicate the functional immaturity of immune cells of the patients with type 2 diabetes mellitus and give reason to consider the changes of membrane properties of leukocytes as one of the pathogenetic mechanisms of immune cells for developing hyperglycemic conditions.
Series on Biomechanics, Vol.34, No.4 (2020), 18-25
Ключови думи: adhesion force; elastic properties; Leukocytes; surface potential; type 2 diabetes mellitus
Дата на публикуване: 2020-12-11
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